Hotspot

clpP

LSC · 70364-72630 · 2267 bp

Pi 0.00575

MarkerSeek score 51.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2267
variable_sites: 13
pi: 0.00575
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATAGAGCGGGTTGGCTTAGA	TGAAGACAATAAAACCATTGTTACGT	50.0	30.8	58.2	58.1	2433-2437 bp	1.0	40.7
clpP_p2	TAGAGCGGGTTGGCTTAGAT	TGAAGACAATAAAACCATTGTTACGT	50.0	30.8	58.2	58.1	2432-2436 bp	1.0	40.7
clpP_p3	CGGGTTGGCTTAGATCGATC	TGAAGACAATAAAACCATTGTTACGT	55.0	30.8	58.2	58.1	2427-2431 bp	1.0	40.7
clpP_p4	ATAGAGCGGGTTGGCTTAGA	AGACAATAAAACCATTGTTACGT	50.0	30.4	58.2	54.8	2430-2434 bp	1.0	40.7
clpP_p5	TAGAGCGGGTTGGCTTAGAT	AGACAATAAAACCATTGTTACGT	50.0	30.4	58.2	54.8	2429-2433 bp	1.0	40.7