Hotspot

ycf1-rpl32

SSC · 110843-111305 · 463 bp

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Pi 0.033
MarkerSeek score 76.50
Primer available yes
Label ycf1-rpl32

Feature Metadata

label_name
ycf1-rpl32
feature_type
igs
region
SSC
length_bp
463
variable_sites
15
pi
0.033
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-rpl32_p1 GGGGGAGGTTTGTGATGATT GCTTTTACTGCCTCTAAAGATCC 50.0 43.5 57.5 57.3 630-667 bp 1.0 48.6
ycf1-rpl32_p2 GGGGGAGGTTTGTGATGATT AGAAAAAGCTTTTACTGCCTCT 50.0 36.4 57.5 56.2 637-674 bp 1.0 48.0
ycf1-rpl32_p3 GGGGGAGGTTTGTGATGATT AAGAAAAAGCTTTTACTGCCTCT 50.0 34.8 57.5 56.9 638-675 bp 1.0 45.6
ycf1-rpl32_p4 GGGGGAGGTTTGTGATGATT AGAAAAAGCTTTTACTGCCTC 50.0 38.1 57.5 54.7 637-674 bp 1.0 43.9
ycf1-rpl32_p5 GGGGGAGGTTTGTGATGATT GAAAAAGCTTTTACTGCCTCT 50.0 38.1 57.5 54.7 636-673 bp 1.0 43.9