Hotspot

rbcL-accD

LSC · 56579-57382 · 804 bp

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Pi 0.0124
MarkerSeek score 58.40
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
804
variable_sites
10
pi
0.0124
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 CTAGCAAATGGAGCCCTGAG TGTCCAAGAGCATCAAGACT 55.0 45.0 58.0 56.5 1003 bp 1.0 68.3
rbcL-accD_p2 TGAAGCTAGCAAATGGAGCC TGTCCAAGAGCATCAAGACT 50.0 45.0 58.2 56.5 1008 bp 1.0 66.6
rbcL-accD_p3 TTTCGACCCAGTGGATAAGC TGTCCAAGAGCATCAAGACT 50.0 45.0 57.6 56.5 942 bp 1.0 65.4
rbcL-accD_p4 GCTGCTTGTGAAGTCTGGAA TGTCCAAGAGCATCAAGACT 50.0 45.0 58.4 56.5 977 bp 1.0 65.3
rbcL-accD_p5 CTAGCAAATGGAGCCCTGAG ATGTCCAAGAGCATCAAGACT 55.0 42.9 58.0 57.0 1004 bp 1.0 64.5