Hotspot

psaA-ycf3

LSC · 43102-43675 · 574 bp

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Pi 0.00873
MarkerSeek score 63.40
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
574
variable_sites
5
pi
0.00873
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 CCCATTCCTCGAAAGACGTT ATAGCGCTTACTCCAGGGAA 50.0 50.0 57.9 58.2 722-737 bp 1.0 78.2
psaA-ycf3_p2 CCCATTCCTCGAAAGACGTT TAGCGCTTACTCCAGGGAAT 50.0 50.0 57.9 58.2 721-736 bp 1.0 78.2
psaA-ycf3_p3 CCCATTCCTCGAAAGACGTT GTTCGATCAAGCTGCTGAGT 50.0 50.0 57.9 58.3 756-771 bp 1.0 77.6
psaA-ycf3_p4 CCCATTCCTCGAAAGACGTT GCTATAGCGCTTACTCCAGG 50.0 55.0 57.9 57.6 725-740 bp 1.0 77.1
psaA-ycf3_p5 CCCATTCCTCGAAAGACGTT CTTGGTTCGATCAAGCTGCT 50.0 50.0 57.9 58.6 760-775 bp 1.0 75.4