Hotspot

petN-psbM

LSC · 29822-30677 · 856 bp

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Pi 0.0153
MarkerSeek score 68.60
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
856
variable_sites
13
pi
0.0153
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 GATAGGGGCATCATTCGCAT ACGTCAAAACAGTCAGCCAA 50.0 45.0 57.9 58.3 984-993 bp 1.0 78.8
petN-psbM_p2 GATAGGGGCATCATTCGCAT ATTGCTACTGCGCTGTTCAT 50.0 45.0 57.9 58.3 1036-1045 bp 1.0 78.7
petN-psbM_p3 ATAGGGGCATCATTCGCATG ACGTCAAAACAGTCAGCCAA 50.0 45.0 58.2 58.3 983-992 bp 1.0 78.4
petN-psbM_p4 GGGCATCATTCGCATGGATA ACGTCAAAACAGTCAGCCAA 50.0 45.0 58.2 58.3 979-988 bp 1.0 78.4
petN-psbM_p5 ATAGGGGCATCATTCGCATG ATTGCTACTGCGCTGTTCAT 50.0 45.0 58.2 58.3 1035-1044 bp 1.0 78.3