Hotspot

rpoB-trnC-GCA

LSC · 27278-28843 · 1566 bp

Back to Acampe

Pi 0.00717
MarkerSeek score 64.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1566
variable_sites
11
pi
0.00717
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AATTCTTCCGTCAAGCCCTG CCGGGGATAAAGGATTTGCA 50.0 50.0 58.2 57.9 1689-1721 bp 1.0 80.1
rpoB-trnC-GCA_p2 AATTCTTCCGTCAAGCCCTG ACCGGGGATAAAGGATTTGC 50.0 50.0 58.2 57.9 1690-1722 bp 1.0 80.1
rpoB-trnC-GCA_p3 AATTCTTCCGTCAAGCCCTG TCGAACCGGGGATAAAGGAT 50.0 50.0 58.2 58.2 1694-1726 bp 1.0 79.0
rpoB-trnC-GCA_p4 AATTCTTCCGTCAAGCCCTG TTCGAACCGGGGATAAAGGA 50.0 50.0 58.2 58.4 1695-1727 bp 1.0 77.2
rpoB-trnC-GCA_p5 AAATTCTTCCGTCAAGCCCT CCGGGGATAAAGGATTTGCA 45.0 50.0 57.4 57.9 1690-1722 bp 1.0 76.8