Hotspot

rpoC1-rpoB

LSC · 24039-24064 · 26 bp

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Pi 0.0385
MarkerSeek score 74.60
Primer available yes
Label rpoC1-rpoB

Feature Metadata

label_name
rpoC1-rpoB
feature_type
igs
region
LSC
length_bp
26
variable_sites
1
pi
0.0385
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1-rpoB_p1 GGGAAACTGGTCCAATTCGA AGAGGGTGCTCCAGAATCTT 50.0 50.0 57.8 58.0 193 bp 1.0 78.6
rpoC1-rpoB_p2 GGGAAACTGGTCCAATTCGA TTGCTCGTTCGAGAACTACG 50.0 50.0 57.8 58.0 168 bp 1.0 78.6
rpoC1-rpoB_p3 TTGTTGAGGGGAAACTGGTC TTGCTCGTTCGAGAACTACG 50.0 50.0 57.6 58.0 176 bp 1.0 77.3
rpoC1-rpoB_p4 TTGTTGAGGGGAAACTGGTC AGAGGGTGCTCCAGAATCTT 50.0 50.0 57.6 58.0 201 bp 1.0 77.2
rpoC1-rpoB_p5 GGGTTTTGTCACCTCTCCAA AGAGGGTGCTCCAGAATCTT 50.0 50.0 57.6 58.0 264 bp 1.0 77.1