Hotspot

rps16-trnQ-UUG

LSC · 6091-6961 · 871 bp

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Pi 0.0138
MarkerSeek score 61.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
871
variable_sites
12
pi
0.0138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 ATCCACAGCCAATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 965-967 bp 1.0 80.0
rps16-trnQ-UUG_p2 ATCCACAGCCAATCATGTCC GACCAAAAAGAAATGGGGCG 50.0 50.0 57.9 57.9 1026-1028 bp 1.0 80.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 942-944 bp 1.0 79.5
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GACCAAAAAGAAATGGGGCG 45.0 50.0 58.1 57.9 1003-1005 bp 1.0 79.4
rps16-trnQ-UUG_p5 CCACAGCCAATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 963-965 bp 1.0 79.3