Hotspot

rpl23

IRa · 174169-174468 · 300 bp

Pi 0.23

MarkerSeek score 61.10

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: IRa
length_bp: 300
variable_sites: 25
pi: 0.23
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.25
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	GAATGGTTAAAGCGCCCAAC	CACTTGACTGTCTACGGCTC	50.0	55.0	58.0	58.0	588 bp	0.333333	53.1
rpl23_p2	GAATGGTTAAAGCGCCCAAC	CCACAACGATGCTGTCCATA	50.0	50.0	58.0	58.0	635 bp	0.333333	53.1
rpl23_p3	GAATGGTTAAAGCGCCCAAC	TCTGTCGGTGCTTGAAACAA	50.0	45.0	58.0	58.0	1143 bp	0.333333	53.0
rpl23_p4	GAATGGTTAAAGCGCCCAAC	TTCTGTCGGTGCTTGAAACA	50.0	45.0	58.0	58.0	1144 bp	0.333333	53.0
rpl23_p5	GTTGAACCCTGTGAATTGCG	CACTTGACTGTCTACGGCTC	50.0	55.0	57.9	58.0	897 bp	0.333333	52.8