Hotspot

clpP

LSC · 77225-79431 · 2207 bp

Back to Acacia

Pi 0.113
MarkerSeek score 81.90
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2207
variable_sites
345
pi
0.113
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 CTAGTACATGTTCCTCGGCG TGGACAGAAAGTAAACGGCC 55.0 50.0 57.9 58.1 2748-2990 bp 0.666667 76.7
clpP_p2 CTAGTACATGTTCCTCGGCG AAACGGCCGGGATCATTTAA 55.0 45.0 57.9 57.9 2736-2978 bp 0.666667 76.6
clpP_p3 AGTCGCACATACACCCTAGT AAACGGCCGGGATCATTTAA 50.0 45.0 58.2 57.9 2751-2993 bp 0.666667 76.3
clpP_p4 TGACATTTCGGATTGGCTGT TGGACAGAAAGTAAACGGCC 45.0 50.0 57.8 58.1 2696-2938 bp 0.666667 76.2
clpP_p5 CTAGTACATGTTCCTCGGCG ACCAAGGCAAACCCATGAAA 55.0 45.0 57.9 58.2 2696-2938 bp 0.666667 76.1