Hotspot

accD

LSC · 63921-65501 · 1581 bp

Back to Acacia

Pi 0.0614
MarkerSeek score 81.00
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
1581
variable_sites
145
pi
0.0614
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 TCCTTTCGTTTCGTGTTGGA CCATTGCAATTGCCGGAAAT 45.0 45.0 57.7 58.0 2743-2828 bp 0.666667 72.9
accD_p2 TCCTTTCGTTTCGTGTTGGA AAGAAGCCATTGCAATTGCC 45.0 45.0 57.7 57.9 2749-2834 bp 0.666667 72.3
accD_p3 TGACACAACAGGGGGTAAAC CCATTGCAATTGCCGGAAAT 50.0 45.0 57.6 58.0 2599 bp 0.333333 50.3
accD_p4 TGACACAACAGGGGGTAAAC AAGAAGCCATTGCAATTGCC 50.0 45.0 57.6 57.9 2605 bp 0.333333 49.7
accD_p5 AATTTGACACAACAGGGGGT CCATTGCAATTGCCGGAAAT 45.0 45.0 57.5 58.0 2603 bp 0.333333 49.4