Hotspot

trnI-CAU-ycf2

IRb · 94425-94512 · 88 bp

Back to Acacia

Pi 0.0833
MarkerSeek score 77.90
Primer available yes
Label trnI-CAU-ycf2

Feature Metadata

label_name
trnI-CAU-ycf2
feature_type
igs
region
IRb
length_bp
88
variable_sites
11
pi
0.0833
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU-ycf2_p1 GTTGGGCGCTTTAACCATTC CCAGCGACGGTTTCATTAGA 50.0 50.0 58.0 58.0 719-731 bp 1.0 83.4
trnI-CAU-ycf2_p2 GTTGGGCGCTTTAACCATTC TCCAGCGACGGTTTCATTAG 50.0 50.0 58.0 58.0 720-732 bp 1.0 83.4
trnI-CAU-ycf2_p3 CACTTGACTGTCTACGGCTC CCAGCGACGGTTTCATTAGA 55.0 50.0 58.0 58.0 1293-1573 bp 1.0 83.2
trnI-CAU-ycf2_p4 CACTTGACTGTCTACGGCTC TCCAGCGACGGTTTCATTAG 55.0 50.0 58.0 58.0 1294-1574 bp 1.0 83.2
trnI-CAU-ycf2_p5 CCACAACGATGCTGTCCATA CCAGCGACGGTTTCATTAGA 50.0 50.0 58.0 58.0 1340-1620 bp 1.0 83.0