Hotspot

ndhC-trnV-UAC

LSC · 56519-57572 · 1054 bp

Back to Acacia

Pi 0.0432
MarkerSeek score 82.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1054
variable_sites
58
pi
0.0432
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGCCCTTTATTAATCGGGG AAAAGATGCACGTTGGGTCT 50.0 45.0 57.3 58.0 1131-1272 bp 1.0 82.3
ndhC-trnV-UAC_p2 TGGCCCTTTATTAATCGGGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1060-1201 bp 1.0 81.1
ndhC-trnV-UAC_p3 TGGCCCTTTATTAATCGGGG GATGCACGTTGGGTCTGTAA 50.0 50.0 57.3 58.2 1127-1268 bp 1.0 80.9
ndhC-trnV-UAC_p4 TGGCCCTTTATTAATCGGGG ATGCACGTTGGGTCTGTAAA 50.0 45.0 57.3 57.7 1126-1267 bp 1.0 80.4
ndhC-trnV-UAC_p5 TGGCCCTTTATTAATCGGGG ACGTTGGGTCTGTAAAGCAA 50.0 45.0 57.3 57.7 1122-1263 bp 1.0 79.8