Hotspot

trnS-GCU-trnG-UCC

LSC · 9291-10367 · 1077 bp

Back to Acacia

Pi 0.0481
MarkerSeek score 85.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1077
variable_sites
64
pi
0.0481
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.91
markerseek_score
85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 1090-1189 bp 1.0 82.8
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGAATCAAACGAGGGATCCC 50.0 50.0 58.0 57.3 1089-1188 bp 1.0 82.8
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA TGAATCAAACGAGGGATCCC 50.0 50.0 58.2 57.3 1144-1243 bp 1.0 81.4
trnS-GCU-trnG-UCC_p4 CAATCCGACGCTTTAGTCCA GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 1058-1157 bp 1.0 80.4
trnS-GCU-trnG-UCC_p5 AATCCGACGCTTTAGTCCAC GGATCCATAGAACGAATCGCA 50.0 47.6 58.0 58.0 1057-1156 bp 1.0 80.4