Hotspot

psbI-trnS-GCU

LSC · 8764-9202 · 439 bp

Back to Acacia

Pi 0.063
MarkerSeek score 80.20
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
439
variable_sites
36
pi
0.063
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 CCTATCTAACGATCCAGGACG TGGACTAAAGCGTCGGATTG 52.4 50.0 57.2 58.0 495-566 bp 1.0 74.6
psbI-trnS-GCU_p2 CCTATCTAACGATCCAGGACG GTGGACTAAAGCGTCGGATT 52.4 50.0 57.2 58.0 496-567 bp 1.0 74.6
psbI-trnS-GCU_p3 CCTATCTAACGATCCAGGACG AAGGGCCCTTTTCAATTGGT 52.4 45.0 57.2 57.9 540-611 bp 1.0 73.3
psbI-trnS-GCU_p4 CCTATCTAACGATCCAGGACG AGGGCCCTTTTCAATTGGTT 52.4 45.0 57.2 57.9 539-610 bp 1.0 73.3
psbI-trnS-GCU_p5 CCTATCTAACGATCCAGGACG TTTTTCGTACCGAGGGTTCG 52.4 50.0 57.2 58.2 458-529 bp 1.0 73.3