Hotspot

rps19

LSC · 88664-88942 · 279 bp

Back to Abutilon

Pi 0.0041

MarkerSeek score 28.50

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 4
pi: 0.0041
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 28.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	ATTCCCTCGACACCAGAAGA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.1	58.1	1132-1226 bp	1.0	85.4
rps19_p2	TTCCCTCGACACCAGAAGAT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.1	58.1	1131-1225 bp	1.0	85.4
rps19_p3	ATTCCCTCGACACCAGAAGA	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.1	58.1	1330-1424 bp	1.0	85.1
rps19_p4	TTCCCTCGACACCAGAAGAT	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.1	58.1	1329-1423 bp	1.0	85.1
rps19_p5	TTCCCTCGACACCAGAAGAT	ATTACCTTCTGGGGAGGTCC	50.0	55.0	58.1	58.1	1411-1505 bp	1.0	84.8