Hotspot

petA-psbJ

LSC · 67216-68284 · 1069 bp

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Pi 0.0153

MarkerSeek score 62.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 48
pi: 0.0153
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAAGCGGTCCTTCCTGATAC	CTTGATGAAATGCGGAGGGA	55.0	50.0	58.1	58.0	1952-2047 bp	1.0	84.0
petA-psbJ_p2	CAAGCGGTCCTTCCTGATAC	TGATGAAATGCGGAGGGAAG	55.0	50.0	58.1	58.0	1950-2045 bp	1.0	84.0
petA-psbJ_p3	CAAGCGGTCCTTCCTGATAC	ACACAATCAAACCCGAACGA	55.0	45.0	58.1	58.0	2175-2271 bp	1.0	83.6
petA-psbJ_p4	CAAGCGGTCCTTCCTGATAC	GAGCCGACAAGGAATTCCAT	55.0	50.0	58.1	58.0	2412-2508 bp	1.0	83.2
petA-psbJ_p5	CAAGCGGTCCTTCCTGATAC	TACAGAGAGCCGACAAGGAA	55.0	50.0	58.1	58.1	2418-2514 bp	1.0	82.9