Hotspot

atpB-rbcL

LSC · 57814-58779 · 966 bp

Back to Abutilon

Pi 0.0121

MarkerSeek score 61.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 966
variable_sites: 33
pi: 0.0121
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTCAAGCGTGGAAACTCCA	AGTCTCTGTTTGTGGTGACA	45.0	45.0	58.0	56.6	954-1079 bp	1.0	75.2
atpB-rbcL_p2	TTTCAAGCGTGGAAACTCCA	AGTCTCTGTTTGTGGTGACAT	45.0	42.9	58.0	57.2	954-1079 bp	1.0	71.4
atpB-rbcL_p3	TTTCAAGCGTGGAAACTCCA	TGCTTTAGTCTCTGTTTGTGGT	45.0	40.9	58.0	57.8	960-1085 bp	1.0	69.0
atpB-rbcL_p4	TTTCAAGCGTGGAAACTCCA	GTCTCTGTTTGTGGTGACAT	45.0	45.0	58.0	55.6	953-1078 bp	1.0	67.0
atpB-rbcL_p5	GGGGAAAGGCTACATCCAGT	AGTCTCTGTTTGTGGTGACA	55.0	45.0	59.1	56.6	1014-1139 bp	1.0	66.8