Hotspot

ndhC-trnV

LSC · 53456-54711 · 1256 bp

Back to Abutilon

Pi 0.0156

MarkerSeek score 63.20

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 1256
variable_sites: 54
pi: 0.0156
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TCATATTCGTAAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	31.8	50.0	53.9	57.8	1226-1316 bp	1.0	47.4
ndhC-trnV_p2	TCATATTCGTAAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	31.8	55.0	53.9	57.5	1225-1315 bp	1.0	47.4
ndhC-trnV_p3	ATCATATTCGTAAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.5	57.8	1227-1317 bp	1.0	47.4
ndhC-trnV_p4	TCATATTCGTAAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.5	57.8	1226-1316 bp	1.0	47.4
ndhC-trnV_p5	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.9	58.7	1214-1304 bp	1.0	47.4