Hotspot

rps4-trnT-UGU

LSC · 47923-48434 · 512 bp

Back to Abutilon

Pi 0.0137

MarkerSeek score 68.00

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 21
pi: 0.0137
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAGATCGCTTCCGGCTCTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	673-797 bp	1.0	84.1
rps4-trnT-UGU_p2	TTTCTAAGATCGCTTCCGGC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	677-801 bp	1.0	83.7
rps4-trnT-UGU_p3	TAAGATCGCTTCCGGCTCTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	641-765 bp	1.0	83.5
rps4-trnT-UGU_p4	TTTCTAAGATCGCTTCCGGC	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.1	57.8	645-769 bp	1.0	83.1
rps4-trnT-UGU_p5	TAAGATCGCTTCCGGCTCTA	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.0	57.5	681-805 bp	1.0	81.1