Hotspot

trnS-GCU-trnS-CGA

LSC · 8556-9381 · 826 bp

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Pi 0.0216
MarkerSeek score 71.10
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
826
variable_sites
49
pi
0.0216
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 CAATCCGACGCTTTAGTCCA ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 876-931 bp 1.0 59.5
trnS-GCU-trnS-CGA_p2 AATCCGACGCTTTAGTCCAC ACGAATCACACTTTTACCACT 50.0 38.1 58.0 55.5 875-930 bp 1.0 59.5
trnS-GCU-trnS-CGA_p3 CGCACAACGGATTAGCAATC ACGAATCACACTTTTACCACT 50.0 38.1 57.9 55.5 891-946 bp 1.0 58.6
trnS-GCU-trnS-CGA_p4 CAATCCGACGCTTTAGTCCA AGAACGAATCACACTTTTACCAC 50.0 39.1 58.0 57.3 879-934 bp 1.0 58.4
trnS-GCU-trnS-CGA_p5 CAATCCGACGCTTTAGTCCA GAACGAATCACACTTTTACCACT 50.0 39.1 58.0 57.3 878-933 bp 1.0 58.4