Hotspot

ndhH-rps15

SSC · 125033-125139 · 107 bp

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Pi 0.0693
MarkerSeek score 85.20
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
SSC
length_bp
107
variable_sites
7
pi
0.0693
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 GGTCCTTTCTTGTAGCTGGG TCGGGAGTCACAAATTCGTT 55.0 45.0 57.9 57.5 151-157 bp 1.0 81.5
ndhH-rps15_p2 GGTCCTTTCTTGTAGCTGGG TTCGGGAGTCACAAATTCGT 55.0 45.0 57.9 57.5 152-158 bp 1.0 81.5
ndhH-rps15_p3 AACACCATGCATTGATGGGT TCGGGAGTCACAAATTCGTT 45.0 45.0 58.1 57.5 198-204 bp 1.0 80.4
ndhH-rps15_p4 AACACCATGCATTGATGGGT TTCGGGAGTCACAAATTCGT 45.0 45.0 58.1 57.5 199-205 bp 1.0 80.4
ndhH-rps15_p5 ATGCATTGATGGGTGGTGAG TCGGGAGTCACAAATTCGTT 50.0 45.0 58.2 57.5 192-198 bp 1.0 79.2