Hotspot

rps3-rps19

LSC · 85803-86295 · 493 bp

Back to Abrus

Pi 0.0647
MarkerSeek score 86.30
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
493
variable_sites
30
pi
0.0647
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 CAAGTCTGAAACCAAGCGGA ACCTACTCTAAACTTCCGAGGA 50.0 45.5 58.4 57.7 560-585 bp 1.0 66.4
rps3-rps19_p2 CAAGTCTGAAACCAAGCGGA CACCTACTCTAAACTTCCGAGG 50.0 50.0 58.4 57.6 561-586 bp 1.0 65.9
rps3-rps19_p3 AGTCTGAAACCAAGCGGATT ACCTACTCTAAACTTCCGAGGA 45.0 45.5 57.4 57.7 558-583 bp 1.0 65.2
rps3-rps19_p4 CAAGTCTGAAACCAAGCGGA ACTCTAAACTTCCGAGGACA 50.0 45.0 58.4 55.5 556-581 bp 1.0 65.2
rps3-rps19_p5 AAGTCTGAAACCAAGCGGAT ACCTACTCTAAACTTCCGAGGA 45.0 45.5 57.4 57.7 559-584 bp 1.0 65.1