Hotspot

rpl36-rps8

LSC · 81840-82309 · 470 bp

Back to Abrus

Pi 0.0779
MarkerSeek score 76.30
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
470
variable_sites
36
pi
0.0779
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 ACGGAGGCCCCTATTTTCAT GGCGGAGAAATTTTATGTTAT 50.0 33.3 58.8 52.3 520-524 bp 1.0 46.7
rpl36-rps8_p2 ACGGAGGCCCCTATTTTCA GGCGGAGAAATTTTATGTTAT 52.6 33.3 58.3 52.3 520-524 bp 1.0 46.7
rpl36-rps8_p3 ACGGAGGCCCCTATTTTCAT GGCGGAGAAATTTTATGTTATA 50.0 31.8 58.8 52.4 520-524 bp 1.0 46.7
rpl36-rps8_p4 ACGGAGGCCCCTATTTTCAT GGCGGAGAAATTTTATGTTATAT 50.0 30.4 58.8 53.0 520-524 bp 1.0 46.7
rpl36-rps8_p5 ACGGAGGCCCCTATTTTCAT GGCGGAGAAATTTTATGTTATATATGG 50.0 33.3 58.8 57.0 520-524 bp 1.0 46.7