Hotspot

rps12-clpP

LSC · 71605-71798 · 194 bp

Back to Abrus

Pi 0.0625
MarkerSeek score 74.80
Primer available yes
Label rps12-clpP

Feature Metadata

label_name
rps12-clpP
feature_type
igs
region
LSC
length_bp
194
variable_sites
12
pi
0.0625
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-clpP_p1 TGACATTTCGGATTGGCTGT AAGCCCACGGAATTGTTGAT 45.0 45.0 57.8 58.1 287-300 bp 1.0 81.6
rps12-clpP_p2 TTTCGGATTGGCTGTCTTGT AAGCCCACGGAATTGTTGAT 45.0 45.0 57.7 58.1 282-295 bp 1.0 81.1
rps12-clpP_p3 TGACATTTCGGATTGGCTGT TTATGTCAGCAGCAGAAGCC 45.0 50.0 57.8 58.3 308-321 bp 1.0 79.9
rps12-clpP_p4 TTCGGATTGGCTGTCTTGTG AAGCCCACGGAATTGTTGAT 50.0 45.0 58.5 58.1 281-294 bp 1.0 79.5
rps12-clpP_p5 TTTCGGATTGGCTGTCTTGT TTATGTCAGCAGCAGAAGCC 45.0 50.0 57.7 58.3 303-316 bp 1.0 79.4