Hotspot

petG-trnW-CCA

LSC · 67819-67945 · 127 bp

Back to Abrus

Pi 0.063
MarkerSeek score 78.10
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
127
variable_sites
8
pi
0.063
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 GTGATCAGCTGGACCTTTGA AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.5 58.0 222 bp 1.0 78.9
petG-trnW-CCA_p2 GTGATCAGCTGGACCTTTGA CAAAACCCGATGTCGTAGGT 50.0 50.0 57.5 57.9 186 bp 1.0 78.9
petG-trnW-CCA_p3 GGTGATCAGCTGGACCTTTG AGCGCTCTTAGTTCAGTTCG 55.0 50.0 58.5 58.0 223 bp 1.0 78.4
petG-trnW-CCA_p4 GGTGATCAGCTGGACCTTTG CAAAACCCGATGTCGTAGGT 55.0 50.0 58.5 57.9 187 bp 1.0 78.4
petG-trnW-CCA_p5 AGGTGATCAGCTGGACCTTT AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.6 58.0 224 bp 1.0 77.7