Hotspot

atpA-trnR-UCU

LSC · 52671-52960 · 290 bp

Back to Abrus

Pi 0.0596
MarkerSeek score 74.60
Primer available yes
Label atpA-trnR-UCU

Feature Metadata

label_name
atpA-trnR-UCU
feature_type
igs
region
LSC
length_bp
290
variable_sites
17
pi
0.0596
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA-trnR-UCU_p1 TGGAACTCTTTTTACTCCAGGA GGTTCAAATCCTATTGGACGC 40.9 47.6 56.7 57.3 353-376 bp 1.0 44.8
atpA-trnR-UCU_p2 TGGAACTCTTTTTACTCCAGGAA GGTTCAAATCCTATTGGACGC 39.1 47.6 57.4 57.3 353-376 bp 1.0 44.8
atpA-trnR-UCU_p3 ATGGAACTCTTTTTACTCCAGGA GGTTCAAATCCTATTGGACGC 39.1 47.6 57.2 57.3 354-377 bp 1.0 44.8
atpA-trnR-UCU_p4 TGGAACTCTTTTTACTCCAGG GGTTCAAATCCTATTGGACGC 42.9 47.6 55.1 57.3 353-376 bp 1.0 44.8
atpA-trnR-UCU_p5 TGGAACTCTTTTTACTCCAGGA TGGATAGGACAGAGGTCTTCT 40.9 47.6 56.7 56.9 388-411 bp 1.0 44.4