Hotspot

psaB-rps14

LSC · 24052-24192 · 141 bp

Back to Abrus

Pi 0.0851
MarkerSeek score 76.00
Primer available yes
Label psaB-rps14

Feature Metadata

label_name
psaB-rps14
feature_type
igs
region
LSC
length_bp
141
variable_sites
12
pi
0.0851
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaB-rps14_p1 GCCTCTACATCGGGCAAATT TGCCTCTTCTTTTCCCTCTG 50.0 50.0 58.3 57.1 209 bp 1.0 75.1
psaB-rps14_p2 TAATTGCCTCTACATCGGGC TGCCTCTTCTTTTCCCTCTG 50.0 50.0 57.5 57.1 214 bp 1.0 73.2
psaB-rps14_p3 GCCTCTACATCGGGCAAATT TTGCCTCTTCTTTTCCCTCTG 50.0 47.6 58.3 57.9 210 bp 1.0 72.8
psaB-rps14_p4 GCCTCTACATCGGGCAAATT GCCTCTTCTTTTCCCTCTGA 50.0 50.0 58.3 56.8 208 bp 1.0 72.8
psaB-rps14_p5 TAATTGCCTCTACATCGGGC TTGCCTCTTCTTTTCCCTCTG 50.0 47.6 57.5 57.9 215 bp 1.0 70.9