Hotspot

ndhJ-trnF-GAA

LSC · 12446-13235 · 790 bp

Back to Abrus

Pi 0.0444
MarkerSeek score 75.80
Primer available yes
Label ndhJ-trnF-GAA

Feature Metadata

label_name
ndhJ-trnF-GAA
feature_type
igs
region
LSC
length_bp
790
variable_sites
33
pi
0.0444
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-trnF-GAA_p1 TGAAATACAAGATGCTCACT CCTCGTGTCACCAGTTCAAA 35.0 50.0 52.2 58.1 809-846 bp 1.0 47.2
ndhJ-trnF-GAA_p2 TGAAATACAAGATGCTCACT GGGATAGCTCAGCTGGTAGA 35.0 55.0 52.2 58.0 845-882 bp 1.0 47.1
ndhJ-trnF-GAA_p3 TGAAATACAAGATGCTCACT CTGGTAGAGCAGAGGACTGA 35.0 55.0 52.2 57.9 833-870 bp 1.0 47.1
ndhJ-trnF-GAA_p4 ATGAAATACAAGATGCTCACT GGGATAGCTCAGCTGGTAGA 33.3 55.0 52.9 58.0 846-883 bp 1.0 47.1
ndhJ-trnF-GAA_p5 TGAAATACAAGATGCTCACT AGAATGGTCGGGATAGCTCA 35.0 50.0 52.2 57.9 854-891 bp 1.0 47.0