Hotspot

trnK-UUU-rbcL

LSC · 4373-5191 · 819 bp

Back to Abrus

Pi 0.0517
MarkerSeek score 81.90
Primer available yes
Label trnK-UUU-rbcL

Feature Metadata

label_name
trnK-UUU-rbcL
feature_type
igs
region
LSC
length_bp
819
variable_sites
39
pi
0.0517
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rbcL_p1 AGTCGCTCTTAAAAGCCGAG AGTCCTGAATTAGCTGCTGC 50.0 50.0 58.0 58.0 926-939 bp 1.0 87.0
trnK-UUU-rbcL_p2 GTCGCTCTTAAAAGCCGAGT AGTCCTGAATTAGCTGCTGC 50.0 50.0 58.3 58.0 925-938 bp 1.0 84.8
trnK-UUU-rbcL_p3 AAGTCGCTCTTAAAAGCCGA AGTCCTGAATTAGCTGCTGC 45.0 50.0 57.5 58.0 927-940 bp 1.0 83.5
trnK-UUU-rbcL_p4 AGTCGCTCTTAAAAGCCGAG GCTGCTGCTTGTGAAGTATG 50.0 50.0 58.0 57.5 914-927 bp 1.0 83.1
trnK-UUU-rbcL_p5 AGTCGCTCTTAAAAGCCGAG TGGAGTCCTGAATTAGCTGC 50.0 50.0 58.0 57.3 929-942 bp 1.0 81.7