Hotspot

trnL-CAA-ycf2

Genome · 113335-114065 · 731 bp

Back to Abies

Pi 0.00237
MarkerSeek score 56.40
Primer available yes
Label trnL-CAA-ycf2

Feature Metadata

label_name
trnL-CAA-ycf2
feature_type
igs
region
Genome
length_bp
731
variable_sites
9
pi
0.00237
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf2_p1 AAATGGTAGACACGCGAGAC CAGCCAATCTCTAACCATCGA 50.0 47.6 58.0 57.5 816-831 bp 1.0 70.2
trnL-CAA-ycf2_p2 AAATGGTAGACACGCGAGAC TCAGCCAATCTCTAACCATCG 50.0 47.6 58.0 57.5 817-832 bp 1.0 70.2
trnL-CAA-ycf2_p3 TGAAATGGTAGACACGCGAG CAGCCAATCTCTAACCATCGA 50.0 47.6 58.0 57.5 818-833 bp 1.0 70.2
trnL-CAA-ycf2_p4 TGAAATGGTAGACACGCGAG TCAGCCAATCTCTAACCATCG 50.0 47.6 58.0 57.5 819-834 bp 1.0 70.2
trnL-CAA-ycf2_p5 TGGTGAAATGGTAGACACGC CAGCCAATCTCTAACCATCGA 50.0 47.6 58.2 57.5 821-836 bp 1.0 68.7