Hotspot

rps12-rps7

Genome · 111271-111322 · 52 bp

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Pi 0.0181
MarkerSeek score 50.20
Primer available yes
Label rps12-rps7

Feature Metadata

label_name
rps12-rps7
feature_type
igs
region
Genome
length_bp
52
variable_sites
3
pi
0.0181
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-rps7_p1 ATGTACGGTTTGGAGGGAGA GCAGTACTTCGTCGTGACAT 50.0 50.0 58.1 58.0 147 bp 1.0 81.0
rps12-rps7_p2 TGTACGGTTTGGAGGGAGAT GCAGTACTTCGTCGTGACAT 50.0 50.0 58.1 58.0 146 bp 1.0 81.0
rps12-rps7_p3 ATGTACGGTTTGGAGGGAGA TTCTGCAGTACTTCGTCGTG 50.0 50.0 58.1 58.0 151 bp 1.0 80.8
rps12-rps7_p4 TGTACGGTTTGGAGGGAGAT TTCTGCAGTACTTCGTCGTG 50.0 50.0 58.1 58.0 150 bp 1.0 80.8
rps12-rps7_p5 TGTGGAAAGCCGTATTCGAC GCAGTACTTCGTCGTGACAT 50.0 50.0 58.3 58.0 176 bp 1.0 78.9