Hotspot

rps15-psaC

Genome · 102555-103810 · 1256 bp

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Pi 0.00247
MarkerSeek score 50.30
Primer available yes
Label rps15-psaC

Feature Metadata

label_name
rps15-psaC
feature_type
igs
region
Genome
length_bp
1256
variable_sites
21
pi
0.00247
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-psaC_p1 TTTGGGCAAACGTAAGCAAC GTACGCATTGGGTACAACCA 45.0 50.0 57.8 58.2 1417-1863 bp 1.0 77.9
rps15-psaC_p2 TTTGGGCAAACGTAAGCAAC ATCTGTGGGACAAGCTCGTA 45.0 50.0 57.8 58.4 1434-1880 bp 1.0 75.9
rps15-psaC_p3 GGGCAAACGTAAGCAACTTT GTACGCATTGGGTACAACCA 45.0 50.0 57.5 58.2 1414-1860 bp 1.0 75.6
rps15-psaC_p4 TGGGCAAACGTAAGCAACTT GTACGCATTGGGTACAACCA 45.0 50.0 58.6 58.2 1415-1861 bp 1.0 74.8
rps15-psaC_p5 TTGGGCAAACGTAAGCAACT GTACGCATTGGGTACAACCA 45.0 50.0 58.6 58.2 1416-1862 bp 1.0 74.8