Hotspot

clpP

LSC · 75027-77241 · 2215 bp

Back to Abelmoschus

Pi 0.00627

MarkerSeek score 53.90

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2215
variable_sites: 27
pi: 0.00627
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GGTGTTAAACCGTTGCTGTT	ACGCTTCCACATCAAAGTGA	45.0	45.0	57.1	57.7	2284-2289 bp	1.0	72.5
clpP_p2	GGTGTTAAACCGTTGCTGTTT	ACGCTTCCACATCAAAGTGA	42.9	45.0	57.8	57.7	2284-2289 bp	1.0	70.1
clpP_p3	GGTGTTAAACCGTTGCTGTT	GACCACTATTACGCTTCCACA	45.0	47.6	57.1	57.8	2294-2299 bp	1.0	64.6
clpP_p4	GGTGTTAAACCGTTGCTGTT	CGCTTCCACATCAAAGTGAA	45.0	45.0	57.1	56.7	2283-2288 bp	1.0	63.7
clpP_p5	GGTGTTAAACCGTTGCTGTT	TACGCTTCCACATCAAAGTGA	45.0	42.9	57.1	57.6	2285-2290 bp	1.0	63.4