Hotspot

rpl32-trnL-UAG

SSC · 119424-120514 · 1091 bp

Back to Abelmoschus

Pi 0.00352

MarkerSeek score 61.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1091
variable_sites: 7
pi: 0.00352
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	CGGCGTTGAAAGCTTTTTCA	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.2	58.0	1206-1234 bp	1.0	79.3
rpl32-trnL-UAG_p2	CGGCGTTGAAAGCTTTTTCA	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.2	58.0	1207-1235 bp	1.0	79.3
rpl32-trnL-UAG_p3	CGGCGTTGAAAGCTTTTTCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	1216-1244 bp	1.0	78.3
rpl32-trnL-UAG_p4	AAGAAAGGATATTGGGCGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.3	58.0	1222-1250 bp	1.0	78.1
rpl32-trnL-UAG_p5	AAGAAAGGATATTGGGCGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.3	58.0	1223-1251 bp	1.0	78.1