Hotspot

psaJ-rpl33

LSC · 71951-72434 · 484 bp

Back to Abelmoschus

Pi 0.00517

MarkerSeek score 61.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 484
variable_sites: 5
pi: 0.00517
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCCCGGATGCGTTAACATT	ATACCTGGAAATGCCCGTTG	45.0	50.0	57.9	58.2	617-630 bp	1.0	79.0
psaJ-rpl33_p2	GTTTCTTCCCGGATGCGTTA	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	622-635 bp	1.0	77.7
psaJ-rpl33_p3	TTCTTCCCGGATGCGTTAAC	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	620-633 bp	1.0	77.7
psaJ-rpl33_p4	CGGATGCGTTAACATTCCCT	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	613-626 bp	1.0	77.2
psaJ-rpl33_p5	CTTCCCGGATGCGTTAACAT	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	618-631 bp	1.0	77.2