Hotspot

atpB-rbcL

LSC · 58451-59619 · 1169 bp

Back to Abelmoschus

Pi 0.0119

MarkerSeek score 68.30

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1169
variable_sites: 21
pi: 0.0119
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTCAAGCGTGGAAACTCCA	ACTCTTTAACACCGGCTTGG	45.0	50.0	58.0	58.1	1276-1433 bp	1.0	81.4
atpB-rbcL_p2	TTTCAAGCGTGGAAACTCCA	TTAACACCGGCTTGGAATCC	45.0	50.0	58.0	58.2	1271-1428 bp	1.0	80.9
atpB-rbcL_p3	TTTCAAGCGTGGAAACTCCA	CTCTTTAACACCGGCTTGGA	45.0	50.0	58.0	57.8	1275-1432 bp	1.0	80.9
atpB-rbcL_p4	TTTCAAGCGTGGAAACTCCA	GCTTGGAATCCAACACCTGT	45.0	50.0	58.0	58.4	1262-1419 bp	1.0	79.3
atpB-rbcL_p5	TTTCAAGCGTGGAAACTCCA	TCTTTAACACCGGCTTGGAA	45.0	45.0	58.0	57.4	1274-1431 bp	1.0	77.2