Hotspot

ndhC-trnV-UAC

LSC · 54140-55369 · 1230 bp

Back to Abelmoschus

Pi 0.0115

MarkerSeek score 71.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1230
variable_sites: 27
pi: 0.0115
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCCTTTCTAATCGGGGCTA	TGGGTCTTTGAAATAGGCCG	50.0	50.0	57.8	57.9	1432-1487 bp	1.0	82.0
ndhC-trnV-UAC_p2	CGGGGCTAAAACTCCAGAAA	TGGGTCTTTGAAATAGGCCG	50.0	50.0	57.8	57.9	1420-1475 bp	1.0	82.0
ndhC-trnV-UAC_p3	ACCCTTTCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1374-1429 bp	1.0	81.6
ndhC-trnV-UAC_p4	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1362-1417 bp	1.0	81.6
ndhC-trnV-UAC_p5	ATCGGGGCTAAAACTCCAGA	TGGGTCTTTGAAATAGGCCG	50.0	50.0	58.4	57.9	1422-1477 bp	1.0	80.2