Hotspot

rps4-trnT-UGU

LSC · 48998-49601 · 604 bp

Back to Abelmoschus

Pi 0.00368
MarkerSeek score 63.60
Primer available yes
Label rps4-trnT-UGU

Feature Metadata

label_name
rps4-trnT-UGU
feature_type
igs
region
LSC
length_bp
604
variable_sites
4
pi
0.00368
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnT-UGU_p1 TAAGATCGCTTCCGGCTCTA CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.0 57.9 764-797 bp 1.0 80.8
rps4-trnT-UGU_p2 TTTCTAAGATCGCTTCCGGC CTCAGAGGTTAGAGCATCGC 50.0 55.0 58.1 57.9 768-801 bp 1.0 80.5
rps4-trnT-UGU_p3 TAAGATCGCTTCCGGCTCTA GGTTCGATTCCGATAGCTGG 50.0 55.0 58.0 58.2 723-756 bp 1.0 80.3
rps4-trnT-UGU_p4 TAAGATCGCTTCCGGCTCTA ATGGTCATCGGTTCGATTCC 50.0 50.0 58.0 57.8 732-765 bp 1.0 80.0
rps4-trnT-UGU_p5 TTTCTAAGATCGCTTCCGGC GGTTCGATTCCGATAGCTGG 50.0 55.0 58.1 58.2 727-760 bp 1.0 79.9