Hotspot

trnK-UUU-rps16

LSC · 4646-5535 · 890 bp

Back to Abelmoschus

Pi 0.0064

MarkerSeek score 64.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 890
variable_sites: 11
pi: 0.0064
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AGTCGCACTTAAAAGCCGAG	AAAAGGAGCTCAACCCACAG	50.0	50.0	58.6	58.0	1029-1077 bp	1.0	76.7
trnK-UUU-rps16_p2	TAGTCGCACTTAAAAGCCGA	AAAAGGAGCTCAACCCACAG	45.0	50.0	57.3	58.0	1030-1078 bp	1.0	75.4
trnK-UUU-rps16_p3	TCGCACTTAAAAGCCGAGTA	AAAAGGAGCTCAACCCACAG	45.0	50.0	57.3	58.0	1027-1075 bp	1.0	75.4
trnK-UUU-rps16_p4	CGCACTTAAAAGCCGAGTAC	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.2	58.0	1026-1074 bp	1.0	75.0
trnK-UUU-rps16_p5	GCTAGTCGCACTTAAAAGCC	AAAAGGAGCTCAACCCACAG	50.0	50.0	57.2	58.0	1032-1080 bp	1.0	74.8