Hotspot

rpoC2

LSC · 17651-21829 · 4179 bp

Back to Abeliophyllum

Pi 0.0134

MarkerSeek score 61.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4179
variable_sites: 56
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TACGACACGGATCGGGATTA	CCATTTTCCTGGAGAGACCG	50.0	55.0	58.0	58.0	950 bp	0.5	59.5
rpoC2_p2	TACGACACGGATCGGGATTA	GGGTTCAGGATTGCTCTCTG	50.0	55.0	58.0	58.0	458 bp	0.5	59.4
rpoC2_p3	TACGACACGGATCGGGATTA	CCACCAGGAACAGGAAAACA	50.0	50.0	58.0	57.9	898 bp	0.5	59.2
rpoC2_p4	TACGACACGGATCGGGATTA	ACCACCAGGAACAGGAAAAC	50.0	50.0	58.0	57.9	899 bp	0.5	59.2
rpoC2_p5	AATGTTACGACACGGATCGG	CCATTTTCCTGGAGAGACCG	50.0	55.0	58.1	58.0	955 bp	0.5	59.1