Hotspot

ndhH

SSC · 123811-124992 · 1182 bp

Back to Abeliophyllum

Pi 0.000846

MarkerSeek score 59.20

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 1
pi: 0.000846
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	AGGGACAAGCAACCATATGA	TCATGGAAGAATGACTCGGT	45.0	45.0	56.5	56.3	1311 bp	1.0	54.1
ndhH_p2	ACAAGCAACCATATGATCCCA	TCATGGAAGAATGACTCGGT	42.9	45.0	57.3	56.3	1307 bp	1.0	52.8
ndhH_p3	AGGGACAAGCAACCATATGA	TTCATGGAAGAATGACTCGGT	45.0	42.9	56.5	57.0	1312 bp	1.0	52.2
ndhH_p4	ACAAGCAACCATATGATCCCA	TTCATGGAAGAATGACTCGGT	42.9	42.9	57.3	57.0	1308 bp	1.0	50.9
ndhH_p5	AGGGACAAGCAACCATATGAT	TCATGGAAGAATGACTCGGT	42.9	45.0	57.0	56.3	1311 bp	1.0	50.4