Hotspot

rpl20-rps12

LSC · 71699-72490 · 792 bp

Back to Abeliophyllum

Pi 0.00126

MarkerSeek score 54.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 792
variable_sites: 1
pi: 0.00126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.0	58.5	893 bp	1.0	75.5
rpl20-rps12_p2	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	948 bp	1.0	74.8
rpl20-rps12_p3	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	947 bp	1.0	74.8
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	CAGCGTCGAGGAACATGTAC	50.0	55.0	58.0	58.7	856 bp	1.0	74.0
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	910 bp	1.0	73.3