Hotspot

psbM-trnD-GUC

LSC · 31457-32502 · 1046 bp

Back to Abeliophyllum

Pi 0

MarkerSeek score 59.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1046
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.4	58.5	1178-1199 bp	1.0	55.6
psbM-trnD-GUC_p2	AGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.4	58.5	1170-1191 bp	1.0	55.4
psbM-trnD-GUC_p3	AAGCGGTAGGAACTAGAATGA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.2	58.5	1171-1192 bp	1.0	53.9
psbM-trnD-GUC_p4	AGCGGTAGGAACTAGAATGAA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.2	58.5	1170-1191 bp	1.0	53.9
psbM-trnD-GUC_p5	AAGTAGAAAAGCGGTAGGAACT	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.1	58.5	1179-1200 bp	1.0	53.4