Hotspot

rpoB-trnC-GCA

LSC · 28023-29217 · 1195 bp

Back to Abeliophyllum

Pi 0.000837

MarkerSeek score 59.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1195
variable_sites: 1
pi: 0.000837
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CTCATTTCCATCCCCGAGC	CCGGATTTGAACTGGGGAAA	57.9	50.0	58.0	58.1	1278 bp	1.0	71.3
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	1283 bp	1.0	70.8
rpoB-trnC-GCA_p3	TCATTTCCATCCCCGAGCAT	CCGGATTTGAACTGGGGAAA	50.0	50.0	59.2	58.1	1277 bp	1.0	70.2
rpoB-trnC-GCA_p4	CTCATTTCCATCCCCGAGC	AAAAGGATTTGCAGTCCCCC	57.9	50.0	58.0	58.4	1260 bp	1.0	69.1
rpoB-trnC-GCA_p5	ACATTCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.7	58.1	1285 bp	1.0	68.9