Hotspot

trnK-UUU-rps16

LSC · 4408-5251 · 844 bp

Back to Abeliophyllum

Pi 0.00118
MarkerSeek score 59.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
844
variable_sites
1
pi
0.00118
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CGAATTCCTCGCTTCATCCA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.1 58.2 1016 bp 1.0 78.1
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 968 bp 1.0 77.2
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 967 bp 1.0 77.2
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA GGTGCTCAACCTACAGGAAC 50.0 55.0 58.3 58.2 977 bp 1.0 76.3
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GGTGCTCAACCTACAGGAAC 50.0 55.0 57.5 58.2 954 bp 1.0 74.9