Hotspot

rps18

LSC · 73190-73853 · 664 bp

Pi 0.0446

MarkerSeek score 84.70

Primer available yes

Label rps18

Feature Metadata

label_name: rps18
feature_type: gene
region: LSC
length_bp: 664
variable_sites: 66
pi: 0.0446
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18_p1	CGACCGAAATAGGATTTTGGG	GCGTTTGAGTTCGGATTGGA	47.6	50.0	57.0	58.8	757-761 bp	1.0	68.1
rps18_p2	CGACCGAAATAGGATTTTGGG	TGCGTTTGAGTTCGGATTGG	47.6	50.0	57.0	59.1	758-762 bp	1.0	65.8
rps18_p3	ACCGAAATAGGATTTTGGGGA	GCGTTTGAGTTCGGATTGGA	42.9	50.0	57.0	58.8	755-759 bp	0.9	62.7
rps18_p4	GACCGAAATAGGATTTTGGGGA	GCGTTTGAGTTCGGATTGGA	45.5	50.0	58.1	58.8	756-760 bp	0.9	62.1
rps18_p5	ACCGAAATAGGATTTTGGGGA	TGCGTTTGAGTTCGGATTGG	42.9	50.0	57.0	59.1	756-760 bp	0.9	60.4