Hotspot

trnR-ACG-GFC95_pgr004

IRa · 134537-134797 · 261 bp

Back to Abelia

Pi 0.112
MarkerSeek score 91.30
Primer available yes
Label trnR-ACG-GFC95_pgr004

Feature Metadata

label_name
trnR-ACG-GFC95_pgr004
feature_type
igs
region
IRa
length_bp
261
variable_sites
62
pi
0.112
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
91.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-GFC95_pgr004_p1 AGAAGTTCCGGGAGTTACGA AAGCTTCACTGTTCCTTGGG 50.0 50.0 58.1 58.0 2500-2508 bp 1.0 87.2
trnR-ACG-GFC95_pgr004_p2 AGAAGTTCCGGGAGTTACGA CATGTCTGTGAAGATGCGGA 50.0 50.0 58.1 58.0 2550-2558 bp 1.0 87.1
trnR-ACG-GFC95_pgr004_p3 AGAAGTTCCGGGAGTTACGA ATGTCTGTGAAGATGCGGAC 50.0 50.0 58.1 58.0 2549-2557 bp 1.0 87.1
trnR-ACG-GFC95_pgr004_p4 AGAAGTTCCGGGAGTTACGA TAGCGAAATTCCTTGTCGGG 50.0 50.0 58.1 58.0 2639-2647 bp 1.0 87.0
trnR-ACG-GFC95_pgr004_p5 TACGATAGCGGCTAGTCGAT AAGCTTCACTGTTCCTTGGG 50.0 50.0 57.9 58.0 2895-2903 bp 1.0 86.8