Hotspot

trnN-GUU-ndhF

SSC · 114034-115075 · 1042 bp

Back to Abelia

Pi 0.0292
MarkerSeek score 75.10
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
SSC
length_bp
1042
variable_sites
99
pi
0.0292
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 CCACTGAGCTACTGAGGA TGGAAGTGGAAGGAAGGGTA 55.6 50.0 54.8 57.6 1100-1134 bp 1.0 51.2
trnN-GUU-ndhF_p2 CCACTGAGCTACTGAGGA GCTTTTTGTTGCTGTCGGAA 55.6 45.0 54.8 57.8 1154-1188 bp 1.0 50.9
trnN-GUU-ndhF_p3 CCACTGAGCTACTGAGGA AAGGCTTTTTGTTGCTGTCG 55.6 45.0 54.8 57.8 1157-1191 bp 1.0 50.8
trnN-GUU-ndhF_p4 CCACTGAGCTACTGAGGA ACGAAGGCTTTTTGTTGCTG 55.6 45.0 54.8 57.8 1160-1194 bp 1.0 50.8
trnN-GUU-ndhF_p5 CCACTGAGCTACTGAGGA CGAAGGCTTTTTGTTGCTGT 55.6 45.0 54.8 57.8 1159-1193 bp 1.0 50.8