Hotspot

ycf2-trnL-CAA

IRb · 97999-98920 · 922 bp

Back to Abelia

Pi 0.0135
MarkerSeek score 59.30
Primer available yes
Label ycf2-trnL-CAA

Feature Metadata

label_name
ycf2-trnL-CAA
feature_type
igs
region
IRb
length_bp
922
variable_sites
43
pi
0.0135
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnL-CAA_p1 ACAGTATGCGCTTCAAGAGG AAATGGTAGACACGCGAGAC 50.0 50.0 58.0 58.0 1435-1446 bp 0.8 76.2
ycf2-trnL-CAA_p2 ACAGTATGCGCTTCAAGAGG TGAAATGGTAGACACGCGAG 50.0 50.0 58.0 58.0 1437-1448 bp 0.8 76.2
ycf2-trnL-CAA_p3 ACAGTATGCGCTTCAAGAGG CCAACACTCCACCTTTGTCA 50.0 50.0 58.0 57.9 1529-1540 bp 0.8 75.6
ycf2-trnL-CAA_p4 CAAGAGGGTGGTGGAGAGTA AAATGGTAGACACGCGAGAC 55.0 50.0 58.1 58.0 1422-1431 bp 0.6 67.4
ycf2-trnL-CAA_p5 CAAGAGGGTGGTGGAGAGTA TGAAATGGTAGACACGCGAG 55.0 50.0 58.1 58.0 1424-1433 bp 0.6 67.4